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. 1996 Oct 15;16(20):6454–6462. doi: 10.1523/JNEUROSCI.16-20-06454.1996

Fig. 6.

Fig. 6.

BMP4 and Msx-1 expression in the presumptive basilar papilla and macula neglecta. A–Cand F were probed for Msx-1. D andE were probed for BMP4. A andB are transverse sections of a stage 26 embryo (E4.5–E5) showing that the Msx-1 expression was absent in the anterior portion of basilar papilla (arrowhead inA) but present in the posterior portion of the papilla (arrowhead in B). The arrow inA points to part of the positive area in the lagena, which is more obvious in a posterior section shown in B(la). The Msx-1 expression in the papilla decreased over time (stage 27, E5; C) and eventually disappeared with transcripts remaining in the lagena (la) only (see Fig.5B). D and E are transverse sections indicating BMP4 expression in macula neglecta (mn) at stage 29 (E6) and stage 31 (E7), respectively.F is an adjacent section of E probed for Msx-1. Arrowheads in D andE point to positive hybridization signals from the posterior ampulla. The proximal tip of the basilar papilla (bp), which was BMP4-positive, is also shown inD–F. Orientations: D, dorsal; M, medial; hb, hind brain. Scale bars: A–C, 100 μm;D–F = 50 μm.