Fig. 6.
Effect of a β subunit chimera on hSkM1 gating.A, Scaled current recordings obtained from oocytes expressing hSkM1 alone or in the presence of wild-type hβ1 (+β1), wild-type rβ2(+β2), or a chimeric β1/β2subunit (+β1–2). Recording conditions were the same as in Figure 1A. The figure in theinset depicts the proposed membrane topology of wild-type and chimeric β subunits. Thin lines andopen boxes indicate structures from hβ1, whereas thick black lines and filled boxes represent structures from rβ2.B, Alignment of partial amino acid sequences of hβ1, rβ2, and chimeric β1–2. The location of putative transmembrane domains of hβ1 and rβ2 is depicted by thick lines.