Table 2.
Quantitative kinetic data for recovery from inactivation in expressed Na+ channel chimeras
| Channel | +/− hβ1 | τf (msec) | τs(msec) | Fast fraction | (n) | pa |
|---|---|---|---|---|---|---|
| hSkM1 Chimeras | ||||||
| hSkM1 | − | 5.0 ± 2.2 | 1800 ± 727 | 0.37 ± 0.07 | (6) | |
| hSkM1 | + | 4.5 ± 0.8 | 162 ± 22 | 0.77 ± 0.02 | (7) | 2_150 |
| hSkM1–P1 | − | 3.9 ± 0.5 | 380 ± 26 | 0.42 ± 0.05 | (8) | |
| hSkM1–P1 | + | 2.3 ± 0.3 | 150 ± 20 | 0.83 ± 0.03 | (3) | 2_150 |
| hSkM1–P2 | − | 3.2 ± 0.3 | 1300 ± 240 | 0.14 ± 0.07 | (7) | |
| hSkM1–P2 | + | 1.4 ± 0.1 | 250 ± 140 | 0.96 ± 0.02 | (4) | 2_150 |
| hSkM1–P3 | − | 3.4 ± 0.8 | 730 ± 160 | 0.26 ± 0.09 | (3) | |
| hSkM1–P3 | + | 2.3 ± 0.8 | 130 ± 40 | 0.90 ± 0.02 | (4) | 2_150 |
| hSkM1–P4 | − | 205 ± 61 | 1500 ± 170 | 0.26 ± 0.05 | (12) | |
| hSkM1–P4 | + | 3.5 ± 0.7 | 720 ± 80 | 0.65 ± 0.06 | (5) | 2_150 |
| hSkM1–P12 | − | 2.4 ± 0.5 | 430 ± 44 | 0.48 ± 0.04 | (8) | |
| hSkM1–P12 | + | 2.5 ± 0.1 | 110 ± 31 | 0.82 ± 0.01 | (7) | 2_150 |
| hSkM1–P13 | − | 3.9 ± 0.3 | 740 ± 120 | 0.51 ± 0.04 | (6) | |
| hSkM1–P13 | + | 3.0 ± 0.7 | 120 ± 46 | 0.77 ± 0.03 | (4) | 2_150 |
| hSkM1–P23 | − | 3.7 ± 0.6 | 1100 ± 79 | 0.15 ± 0.02 | (9) | |
| hSkM1–P23 | + | 2.8 ± 0.1 | 270 ± 50 | 0.81 ± 0.03 | (7) | 2_150 |
| hSkM1–P24 | − | 6.8 ± 1.1 | 1300 ± 200 | 0.18 ± 0.02 | (8) | |
| hSkM1–P24 | + | 1.5 ± 0.2 | 580 ± 57 | 0.75 ± 0.03 | (4) | 2_150 |
| hSkM1–P34 | − | 5.3 ± 0.8 | 1700 ± 130 | 0.13 ± 0.02 | (7) | |
| hSkM1–P34 | + | 2.6 ± 0.2 | 600 ± 37 | 0.65 ± 0.03 | (11) | 2_150 |
| hSkM1–P14 | − | 3.9 ± 0.5 | 300 ± 42 | 0.37 ± 0.05 | (6) | |
| hSkM1–P14 | + | 3.3 ± 0.2 | 290 ± 33 | 0.29 ± 0.04 | (10) | NS |
| hH1 chimeras | ||||||
| hH1 | − | 6.5 ± 0.6 | 160 ± 45 | 0.81 ± 0.02 | (5) | |
| hH1 | + | 3.7 ± 0.4 | 110 ± 15 | 0.82 ± 0.02 | (8) | NS |
| hH1–P1 | − | 5.6 ± 0.4 | 550 ± 43 | 0.65 ± 0.02 | (6) | |
| hH1–P1 | + | 2.8 ± 0.4 | 310 ± 26 | 0.68 ± 0.03 | (9) | NS |
| hH1–P4 | − | 6.4 ± 0.3 | 140 ± 8 | 0.74 ± 0.01 | (5) | |
| hH1–P4 | + | 3.5 ± 0.6 | 48 ± 10 | 0.80 ± 0.01 | (4) | 2_150 |
| hH1–P14 | − | 5.7 ± 0.6 | 600 ± 78 | 0.66 ± 0.03 | (8) | |
| hH1–P14 | + | 3.1 ± 0.3 | 62 ± 7 | 0.92 ± 0.01 | (12) | 2_150 |
| hH1–P14C | − | 7.4 ± 0.5 | 950 ± 103 | 0.74 ± 0.02 | (10) | |
| hH1–P14C | + | 3.4 ± 0.3 | 139 ± 28 | 0.95 ± 0.01 | (14) | 2_150 |
| hH1–P14N | − | 10.2 ± 0.7 | 1419 ± 149 | 0.69 ± 0.02 | (16) | |
| hH1–P14N | + | 7.1 ± 0.8 | 652 ± 84 | 0.83 ± 0.01 | (8) | 2_150 |
F2_a
Significance level for comparisons between fast fraction in the presence (+) or absence (−) of hβ1. NS, Not significant;
F2_150: p < 0.05; τf, time constant for fast component of Na+channel recovery; τs, time constant for slow component of Na+ channel recovery.