Effect of neonatal MPFC lesions on the expression of D2 receptor mRNA in the striatum and nucleus accumbens assessed byin situ hybridization. Coronal sections were fixed and hybridized with a [α-35S]-labeled D2R probe. Results from the quantitative analysis of in situ hybridization are presented in Table 3. Cpu, Caudate-putamen;Acc, nucleus accumbens; OT, olfactory tubercles; PD39, postnatal day 39; PD60, postnatal day 60.