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. 1996 Sep 15;16(18):5654–5660. doi: 10.1523/JNEUROSCI.16-18-05654.1996

Fig. 1.

Fig. 1.

Induction of apoptosis and its inhibition by Bcl-2 and VAD-FMK in GT1-7 cells. A, Nuclear staining of cells with the fluorescent dye Hoechst 33258 after induction of apoptosis.Panel 1, Untreated, vector control cells; panel 2, vector control cells treated with staurosporine (0.5 μm, 18 hr); panel 3, cells overexpressing Bcl-2 treated with staurosporine (0.5 μm, 18 hr);panel 4, vector control cells preincubated with VAD-FMK (100 μm, 1 hr) before treatment with staurosporine (0.5 μm, 18 hr). Arrow in panel 2 indicates apoptotic nuclei. B, Agarose gel of low molecular weight DNA extracted from cells as indicated. Lane 1, Untreated, vector control cells; lane 2, vector control cells treated with staurosporine (0.5 μm, 18 hr); lane 3, cells overexpressing Bcl-2 treated with staurosporine (0.5 μm, 18 hr); lane 4, vector control cells preincubated with VAD-FMK (100 μm, 1 hr) before treatment with staurosporine (0.5 μm, 18 hr).

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