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Inhibition of the processing of pro-Nedd 2 by Bcl-2. Immunoblots of staurosporine (0.5 μm)-treated GT1-7 cell extracts made at the indicated times using an anti-Nedd 2 antibody. Vector, Extracts from vector control GT1-7 cells; Bcl-2, extracts from Bcl-2-overexpressing GT1-7 cells. Pro-Nedd 2 (∼50 kDa) is indicated by a filled arrow; open arrow indicates a protein band (∼10–12 kDa) that appears to correspond to the C-terminal, smaller subunit of processed Nedd 2.
