Fig. 3.

Detection of replicating and latent virus by IHC and ISH. Balb/c mice were stereotactically inoculated into the caudato-putamen with either 5 × 10⁵ PFU of strain 1716 (5 mice, day 45, postinoculation) or 1 × 10⁶ PFU of strain 17⁺ (3 mice, day 3, postinoculation). They were killed, and the brains were removed and processed as described in Materials and Methods. A, Absence of HSV antigen immunostaining in a representative region of the brain of a strain 1716-infected mouse using a polyclonal anti-HSV-1 antibody.B, LAT⁺ cells were present by ISH in serial sections of all strain 1716-infected mice. C, In strain 1716-infected brains, LATs were localized to the nucleus of neurons as during PNS latency. D, E, HSV-1 antigen-positive neurons and to a lesser extent glial cells were found throughout the brains of 17⁺-infected mice at day 3 after IC inoculation. F, LAT⁺ cells were found in sections of 17⁺-infected mice probed by ISH.G, A small number of LAT⁺ cells also were found in the brainstems of 17⁺-infected mice after corneal inoculation. Scale bar: 800 μm in A, B, F; 80 μm inC; 1.6 mm in D; 160 μm inE; 60 μm in G.