Fig. 4.

Absence of productive cycle HSV-1 gene expression in latently infected mice by in situ hybridization. Balb/c mice were stereotactically inoculated in the caudato-putamen with either 5 × 10⁵ PFU of strain 1716 (3 mice, day 45, postinoculation) or 1 × 10⁶ PFU of strain 17⁺ (3 mice, day 3, postinoculation). They were killed, and the brains were removed and processed as described in Materials and Methods. A–E, Representative coronal sections showing that strain 1716-infected mouse brains were positive for LAT transcripts (A) but negative for ICP4 (B), VP5 (C), gC (D), and SP transcripts (E). F–O, Strain 17⁺-infected mice were positive for LAT (F, K ), ICP4 (G, L), VP5 (H, M ), gC (I, N ), and SP transcripts (J, O). Scale bar: 320 μm in A–J; 160 μm inK–O).