Table 1.
Heme content and microsomal HO enzymatic activity of various kinds of tissues
| Tissue | Heme content (nmol/mg protein)1_a | HO enzymatic activity (pmol bilirubin/mg protein/min)1_b |
|---|---|---|
| Spleen | 13 ± 0.5 | 1430 ± 20 |
| Liver | 1.0 ± 0.02 | 290 ± 4 |
| Whole brain | 1.3 ± 0.1 | 280 ± 5 |
| Olfactory epithelium | 1.5 ± 0.08 | 425 ± 5 |
| Cerebellum | 1.5 ± 0.04 | 310 ± 6 |
F1_a
The heme concentration was determined by fluorescence spectrophotometer.
F1_b
Enzymatic activity was assayed under standard conditions as described in Materials and Methods. Formation of bilirubin was measured spectrophotometrically and expressed as pmol of bilirubin/mg protein/min.
Data are presented as the mean of two to three independent experimental determinations ± SEM.