Fig. 2.

TGF-β induces premature cell death in granule neurons grown in 5 mm K⁺. Cells were cultured as described in Materials and Methods. TGF-β₂ (1 ng/ml) additions were made on DIV1, DIV4, and again on DIV7. A, Viability as assessed by FDA staining. B, Specific LDH release normalized to the value obtained for the 25 mm K⁺control cultures at DIV1. C, DNA fragmentation as described. Bars represent mean ± SEM of three independent determinations.