Fig. 12.

Ang II stimulation of TH activity and TH mRNA in neuronal cultures. A, Fifteen-day-old neuronal cultures grown in 100 mm culture dishes were used for the determination of TH activity after incubation with 100 nm Ang II for indicated time periods, essentially as described previously (Park et al., 1990). Data are mean ± SE (n = 3, * p < 0.05). B and C, Poly(A⁺) RNA from cells treated with 100 nm Ang II in a parallel experiment was isolated and subjected to a semi-quantitative RT-PCR with the use of specific primers for TH, as described in Materials and Methods. B, A representative autoradiogram.C, Density of bands in autoradiograms from three independent experiments was analyzed and quantitated by using SW 5000 Gel Analysis, a PC computer software, and the data were presented as OD units (Lu et al., 1996). Density units were presented as a ratio of TH mRNA product to β-actin mRNA product. Data are mean ± SE. Asterisksindicate significantly different from control (p < 0.05).