Fig. 4.

The effect of Ang II receptor subtype antagonists on the activation of Raf-1 in neuronal cultures. Neuronal cultures were incubated without or with 100 nm Ang II for 10 min in the absence or presence of 10 μmlosartan or PD123319. Active Raf-1 was determined as described in the legend to Figure 3. Top, Representative autoradiogram.Bottom, Data from three experiments are mean ± SE.Asterisks indicate significantly different from control (p < 0.05); double asterisk indicates significantly different from Ang II-treated cells (p < 0.05).