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Co-localization of Ras and Raf-1 in neurons. Neuronal cultures were grown and treated without (a) or with (b) 100 nm Ang II for 15 min at 37°C (Lu et al., 1996). This was followed by double immunocytochemical staining with rabbit anti-Raf-1 and mouse anti-Ras antibodies, followed by staining with rhodamine-labeled anti-rabbit and FITC-labeled anti-mouse IgG. Confocal microscopy was used for image analysis (Lu et al., 1996). Magnification: 5000×.
