Motor neuron markers label large ventral horn neurons in spinal cord slices. Photomicrographs show slices of adult spinal cord stained (as described) with antibody to SMI-32 (A), peripherin (B), CGRP (C), ACh (D), or ChAT (E). For each marker, thebottom panel shows high-power (400×) detail of neurons indicated by arrowhead in top panel. Scale bar, 300 μm.