Fig. 4.

Kainate injury to peripherin(+) and large SMI-32(+) neurons is Ca²⁺-dependent: morphological appearance. Spinal cord cultures were exposed to kainate (100 μm for 10 min), in either the presence of 1.8 mm Ca²⁺ (A, C) or the absence of Ca²⁺ (B, D), and were stained 24 hr later for either peripherin (A, B) or SMI-32 (C, D). Although these submaximal exposures in the presence of Ca²⁺ caused severe damage to many (but not all) labeled neurons, removal of Ca²⁺during the exposure resulted in good preservation of most neurons. Scale bar, 100 μm.