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Bottom. Peripherin(+) neurons and large SMI-32(+) neurons are Co2+(+). Photomicrographs show a peripherin(+) neuron (A, B) and a large SMI-32(+) neuron (C, D) before (A, C) and after (B, D) development of the Co2+stain. Co2+ stains were made light to preserve clarity of pictures. In many neurons, Co2+staining is evident only through nuclear darkening. Arrows(A, C) indicate axonal projections. Scale bar, 100 μm.
