Fig. 3.

AA and LinA, but not phorbol esters or OAG, inhibit Ca²⁺ currents. A, Time course of peak current amplitudes (normalized to the first amplitude) and effects of OAG, PDBu, and AA. These drugs were applied to cells dialyzed for at least 10 min with standard pipette solution (open circles), with 200 U/ml SOD (open squares), or with SOD plus 10 μm of the peptide inhibitor PKCI(19–36) (filled squares). n = 5–6.B, Inhibition of Ca²⁺ currents by 30 μm OAG, 3 μm PDBu, 10 μm SC-10, 100 μm AA, or 100 μm LinA, calculated as % inhibition = 100 − (200 b/a + c), wherea, b, and c are the current amplitudes measured after 60, 120, and 180 sec, respectively, as indicated inA. Neurons were dialyzed for at least 10 min with standard pipette solution (open bars), with SOD (hatched bars), or with SOD plus 10 μm of the peptide inhibitor PKCI(19–36) (filled bars), which significantly attenuates the effects of AA and LinA. Levels of significance for the difference between corresponding bars are indicated. n = 5–6.