Fig. 4.
Translocation of PKCα, ε, and ζ isozymes by phorbol esters and AA. The distribution of protein kinase C isoforms was investigated by immunoblots after a 10 min incubation of cultures with 100 μmAA, with the inactive phorbol ester αPDBU, or with the active phorbol ester βPDBU, both at 1 μm. Cells were lysed as described in Materials and Methods; aliquots of the pellet and supernatant corresponding to 2 × 105 cells were applied to SDS-polyacrylamide gels. Nitrocellulose blots were probed with peptide antisera specific for PKCα (A), PKCε (B), and PKCζ (C). Standard: 10 μg protein from rat neocortical homogenate. Arrows indicate the position of bands that were suppressed when antibodies had been preincubated with the corresponding immunogenic peptide (not shown). Data are representative of three additional experiments performed on different preparations.