Fig. 9.

APPL immunoreactivity in the larval mushroom bodies. A, A semi-horizontal optical section of a wild-type larval CNS, where APPL signal in the neuropil of the mushroom bodies [α (short arrow) and β/γ (long arrow) lobes] is evidenced. B–C, Larval CNSs of genotypes (B) Appl^d; hsp:Appl^s/+ and (C)Appl^d; hsp:Appl^sd/+ were heat-shocked at 37°C for 1 hr, allowed to rest for 4 hr, and immunoprocessed with anti-APPL antibody. Semi-horizontal optical sections are shown. B, APPL^s protein is not enriched in the neuropil of the larval mushroom bodies. C, APPL^sdprotein is highly concentrated in the neuropil of the larval mushroom bodies. For A–C, anterior is to the top. Same magnification was used for all. Scale bar (shown in B): 25 μm.