Table 1.

Quantification of rhGGF2 bound to heparin-Sepharose beads

% of saturation	Picograms of rhGGF2 per bead (±7%)	Equivalent concentration1_a	% of BrdU+ cells in equivalent concentrationsb
6.2	0.75	1.1 ng/ml	10.4 ±  2.8
12.5	1.5	2.2 ng/ml	25.3 ±  5.6
25.0	3.0	4.5 ng/ml	38.5 ±  4.7
50.0	6.0	9.0 ng/ml	53.5 ±  3.2
100.0	12.0	18.0 ng/ml	86.2 ±  6.1

Beads were coated to the indicated degrees of saturation and the amount of bound rhGGF2 immunoassayed (see Materials and Methods for details). Cultures in Figures 2 and 3 received 1500 beads/ml, and the equivalent concentration of free rhGGF2 was applied to the cells in Figure 4.

^F1_aAssumes 1500 beads fully released into 1 ml of medium.

^bAssessed at 40 hours, n = 5 fields (corresponds to Fig. 4).