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. 2019 Jun 14;7(3):10.1128/microbiolspec.psib-0027-2019. doi: 10.1128/microbiolspec.psib-0027-2019

TABLE 1.

Secreted pathogen effectors that localize to the ER or modulate its function

Pathogen(secretion system) Effector Activity Reference(s)
Brucella abortus(VirB T4ASS) BspB Impairs ER-to-Golgi secretory trafficking; interacts with the COG complex in the Golgi and redirects membrane vesicles from the Golgi to Brucella vacuole 91
VceC Localizes to ER (ectopic expression); induces ER stress; interacts with BiP/GRP70 3234
BtpA (TcpB/Btp1) Induces ER stress; binds microtubules; inhibits TLR signaling 7578
BspA Unknown; localizes to ER on ectopic expression 35
BspD Unknown; localizes to ER on ectopic expression 35
BspK Unknown; localizes to ER on ectopic expression 35
Legionella pneumophila(Dot/Icm T4BSS) Lgt1 Inhibits the IRE1 pathway of the UPR; inhibits translation elongation by glucosylation of eukaryotic elongation factor 1A 8284
Lgt2 Inhibits the IRE1 pathway of the UPR; inhibits translation elongation by glucosylation of eukaryotic elongation factor 1A 82, 84, 92
SidE Localizes to the cytoplasmic face of the LCV; regulates ER tubule rearrangement and recruitment of ER markers to the LCV via modulating ubiquitination 64, 9395
SidC Ubiquitin ligase and PI4P binding activity; promotes the association of LCVs with the ER by recruiting ER vesicles 66, 68, 96
SdeA, -B, -C Promote ER reorganization by progressive ADP-ribosylation of ubiquitin and transfer of phosphoribosyl moiety to Rtn4 64, 94, 95, 97
Ceg9 Tethers the LCV to the ER via association with Rtn4 61
SidM/DrrA Recruits Rab1 to LCV; acts as a GEF to recruit vacuoles to the LCV and as a GDF for Rab1; AMPylates Rab1 39, 41, 42, 47, 50, 98100
LidA Interacts with GTP-Rab1 to maintain it in the active conformation 43, 45, 101
SidD Catalyzes AMP release from Rab1 47
AnkX Transfers phosphocholine to Rab1 50
RalF Acts as a GEF to activate ARF 37
Lem3 Reverses activity of AnkX by removing phosphocholine from Rab1 52
SetA Glycosylates Rab1 102, 103
LepB Inactivates Rab1 via RabGAP activity; manipulates phosphoinositide composition of the Legionella-containing vacuole via phosphatidylinositide 4-kinase activity 48, 104, 105
Lpg1137 Cleaves syntaxin 17 at the mitochondrion-associated ER membrane and blocks autophagy 106
RavZ Delipidates Atg8 (LC3-II) at the phagophore to inhibit autophagosome formation 70
LpSpl Sphingosine-1-phosphate lyase disrupts host sphingolipid biosynthesis and inhibits autophagy during infection 71
Chlamydia trachomatis(T3SS) CT229 C. pneumoniae homolog Cpn0585 recruits Rab1 from the ER to the inclusion membrane (effector that recruits Rab1 to C. trachomatis inclusion has yet to be identified) 107
IncD Mediates contact with the ER at MCS via binding to ceramide transfer protein CERT 54, 108
IncV Tethers the C. trachomatis inclusion to the ER via interactions with VAPs 55
MrcA Interacts with the Ca2+ channel inositol-1,4,5-trisphosphate receptor, type 3, to promote release of bacteria from infected cells 109
Coxiella burnetii(Dot/Icm T4BSS) ElpA Localizes to the ER on ectopic expression and blocks secretory traffic 59
Anaplasma spp.(VirB T4ASS) Ats-1 Nucleates autophagosomes by interacting with Beclin and recruitment of DFCP1 and ATG14L to the pathogen-containing vacuole 5, 72
Orientia tsutsugamushi(T1SS) Ank4 Interacts with eukaryotic chaperone Bat3 to transiently impede ER-associated protein degradation 9
Ank9 Destabilizes the ER and Golgi by binding COPB2 and induces ATF4-dependent UPR 27
Rickettsia rickettsii(Rvh T4ASS) RARP-2 Forms membranous structures in association with the ER; contributes to lysis of infected host cells. 11