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. 1996 May 1;16(9):2891–2900. doi: 10.1523/JNEUROSCI.16-09-02891.1996

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Copyright © 1996 Society for Neuroscience

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Fig. 5. — Kinetics of kyotorphin- and GppNHp-evoked⁴⁵Ca²⁺ release from resealed SPM vesicles and involvements of PTX substrate G-proteins and PLC. A, Kyotorphin- and/or GppNHp-evoked⁴⁵Ca²⁺ release were represented as with InsP₃-evoked increase (%) in the ⁴⁵Ca²⁺ release (see Results). B, Double-reciprocal plots of evoked⁴⁵Ca²⁺ release by various combinations of kyotorphin and GppNHp. C, Effects of PTX (50 μg/ml) pretreatments of SPM on 100 μmkyotorphin (plus 10 μm GppNHp)-evoked⁴⁵Ca²⁺ release.D, Blockade of kyotorphin (plus 10 μm GppNHp)-evoked⁴⁵Ca²⁺ release by pretreatments of SPM with various concentrations of PTX. E, Effects of neomycin (0.3 mm) on 100 μm kyotorphin (plus 10 μm GppNHp)-evoked⁴⁵Ca²⁺ release.F, Concentration-dependent inhibition of 100 μm kyotorphin (plus 10 μm GppNHp)-evoked⁴⁵Ca²⁺ release by neomycin.