Table 1.

RT-PCR analysis of mRNA levels during apoptosis in cerebellar granule cells and sympathetic neurons

Gene	K+/serum-deprived granule cells	NGF-deprived sympathetic neurons
cyclophilin	↓	↓
nse	↓	↓
actin	↓	↓
S-100	↔	↔
c-jun	↑	↑
junB	↓	↑
junD	↓	nd
fra-1	↓	↓
fra-2	↓	↓
c-fos	↓	↑
fosB	↓	↑
myb	↔	↑
cyclin D1	↔	↑
cyclin D2	↓	↔
cyclin D3	↓	↓
cdk4	↓	↓
cdk5	↓	↓
p53	↓	↓
MKP-1	↓	↑
transin	↔	↑
collagenase	↔	↑

See Figure 4 for examples of data used to generate this table. Arrows indicate whether the mRNA levels for a given gene increased, decreased, or remained relatively constant. cyclophilin, neuron-specific enolase (nse), and actin are examples of housekeeping genes. c-jun, junB, junD, fra-1, fra-2, c-fos, and fos B are AP-1 transcription factors (Angel and Karin, 1991). c-myb is a transcriptional regulator that is elevated during late G1 and linked with the G1–S-phase cell cycle transition (Gewirtz et al., 1989). cyclin D1, cyclin D2, cyclin D3, cyclin-dependent kinase 4 (cdk4), and cyclin-dependent kinase 5 (cdk5) are regulators of the cell cycle (Pines, 1995).p53 is a tumor suppressor gene associated with PCD (Freeman et al., 1993). MKP-1 inhibits signal transduction through the mitogen-activated protein kinase pathway (Sun et al., 1993).transin and collagenase are extracellular matrix proteases (Matrisian, 1994). nd, Data were not determined. Data from sympathetic neurons are based on Estus et al., 1994 andFreeman et al., 1994.