Typical examples of neurite outgrowth from isolated axons. Axons, which had detached from their cell bodies during plating, sprouted neurites within the first 24 hr (A, C, E), but there was little subsequent growth. The axons were followed for 22 d (B), 9 d (D), and 4 d (F), respectively; shortly thereafter, each disintegrated. Scale bar, 100 μm.