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The in vitro generation of spheres derived from isolated cells of the adult spinal cord is greatest in theLumbar/Sacral segment. The number of spheres generated in the presence of EGF+bFGF in vitro was determined for the three regions of the spinal cord indicated and was normalized to the length of spinal cord tissue dissected. The data represent the mean ± SEM of duplicate determinations in six independent culture preparations.
