Table 1.

Multipotency of primary and renewed adult thoracic spinal cord stem cell-derived spheres

Experimental protocol	Number of independent primary cultures	Frequency of spheres containing neurons, astrocytes, and oligodendrocytes
Primary culture	23	106 /106
Single cell culture	6	11 /11
Single sphere dissociation	15	79 /79

The experimental protocols correspond to those outlined schematically in Figure 5. Indirect immunocytochemistry for the three neural antigens is described in Materials and Methods.