Fig. 4.
Expression of mRNA of three Ca2+channel β subunits in PC12 cells. A, RT-PCR from PC12 cell total RNA with β1, β2, and β3 primers. Left panel, PCR products obtained from PC12 cDNA, control β1, β2, β3, and β4 cDNA with β1primers. Middle panel, PCR products amplified with β2 primers from the same DNA samples as left panel.Right panel, PCR products amplified with β3 primers from the same DNA samples as left panel.B, Alignment of β1 sequence of PC12 RT-PCR product with rat β1b sequence. The different nucleotides are in italics, and dots represent identical nucleotides.