Fig. 3.

Kainic acid increases the BDNF mRNA content of hippocampal neurons in the presence of CHX. Dark-field photomicrographs showing the autoradiographic localization of pan-BDNF cRNA in situ hybridization in sections through control cultured hippocampus (A) and cultured hippocampus treated with kainic acid in the presence of CHX (B). Open arrow indicates the zone of stratum granulosum measured for quantitative analysis presented in Figure 4. In comparison with the control explant (A), labeling of stratum granulosum (sg) is markedly greater in the slice treated with kainic acid (B). In both cases, hybridization is limited to cells in the neuronal lamina and hilus (h).sp, Stratum pyramidale; asteriskindicates artifact in A. Scale bar, 400 μm.