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. Author manuscript; available in PMC: 2019 Jun 17.
Published in final edited form as: Nat Struct Mol Biol. 2018 Oct 29;25(11):1047–1058. doi: 10.1038/s41594-018-0142-5

Figure 4: CRISPR/Cas9 mediated SLFN11 gene knockout confers significant resistance to CPT-induced apoptosis on cells without affecting cell proliferation.

Figure 4:

a, Relative viability of FG cells and FG SLFN11 knockout cells was measured by MTS assay after 48 hours of CPT or DMSO treatment (biological replicates, mean ± s.d., n = 3). b, Knockout of SLFN11 expression in FG cells by CRISPR/Cas9 technique and its effect on ATR/ATM protein expression upon 40 nM CPT or DMSO treatment as determined by immunoblotting. c, As in a, except with HEK293 cells. d, As in b, except with HEK293 cells. Cells were analyzed after 24 hours of 40 nM CPT or DMSO treatment. e, Proliferation of FG and FG SLFN11 knockout cells. f, Relative Caspase-3/7 activity after 24 hours CPT treatment. g, Microscopic images of cell cultures after 24 hours CPT treatment. Uncropped images are shown in Supplementary Data Set 1.