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. Author manuscript; available in PMC: 2019 Jun 17.
Published in final edited form as: Nat Struct Mol Biol. 2018 Oct 29;25(11):1047–1058. doi: 10.1038/s41594-018-0142-5

Figure 8: Gapmers antisense oligonucleotides directed at tRNA-Leu-TAA sensitize intrinsically SLFN11-deficient MIA Paca-2 cells to CPT-induced apoptosis.

Figure 8:

12 hours after transfection with control Gapmer (N.C.) or Gapmers against tRNA-Leu-TAA (12.5 nM), MIA Paca-2 cells were treated with DMSO or 200 nM CPT. a, Total RNA for Northern blots was collected after 12 hours CPT treatment. Protein samples for immunoblotting were collected after 36 hours CPT treatment. Numbers indicate quantified band intensity relative to DMSO treated, Gapmer N.C.-transfected control cell samples. 5.8s rRNA served as control for Northern blots. GAPDH served as control for immunoblotting. b, Relative Caspase-3/7 activity was measured after 24 hours CPT treatment. c, 48 or 72 hours after CPT exposure, relative cell viabilities were determined by MTS assay. (b, c, biological replicates, mean ± s.d., n = 3. Both relative to DMSO treated, Gapmer N.C. transfected control cell samples.) d, Microscopic images of cell cultures after 24 hours CPT treatment. Uncropped images are shown in Supplementary Data Set 1.