Fig. 1.

Kv1.1 transcripts expressed in mature neurons.A, In three experiments, 11 neurons were sampled for Kv1.1 and EF-1α mRNA. Although all are positive for EF-1α (319 bp,bottom), only three are positive for Kv1.1 transcripts (278 bp, top). + and − indicate reactions in which reverse transcriptase (RT) was present or absent, respectively. MC, Reaction mixture control; RC, recording solution control. Brain and spinal RNA serve as positive controls. Reactions in which RT was absent constitute negative controls. B, Restriction digestion with HpaI produces the predicted two fragments, 146 bp and 132 bp in length. This site is present in Kv1.1 products amplified from single spinal neurons, from brain, and from spinal RNA. U, Uncut fragments;C, cut fragments.