Table 1.
Oligonucleotide primers used for RT-PCR assay
| Gene | Sequence | Nucleotides | Reference |
|---|---|---|---|
| Kv1.1 (nested) | F1 GCCATCGCTGGTGTGCTGACA | 1159-1179 | Ribera and Nguyen (1993) |
| F2 CTAACAGTGATCTGAGTCGACG | 1292-1313 | ||
| R1 CCCTAATTTGTACTAGGCCTC | 1571-1551 | ||
| Kv2.2 (nested) | F1 GGATTTACCCTTAGACGGAGT | 973-993 | Burger and Ribera (1996) |
| R2 GCTTCTCTTCTCTTTATTGCC | 1316-1296 | ||
| R1 CCATTTTATTAGACCCATCTTCTG | 1438-1415 | ||
| EF-1α (nested) | F1 CTTCTCAGACTACCCTCCTCT | 1292-1312 | Krieg et al. (1989) |
| R2 GCCACAAACAAATGTGTCCAC | 1610-1590 | ||
| R1 TGGTCTCAAATTTGGTGACAG | 1693-1673 |
F and R refer to forward and reverse primers. Nucleotide numbers refer to positions within published sequences, and bold numbers border nested fragments that were amplified in the second PCR. The published length of the nested sequence of Kv1.1 is 280 bases; however, sequencing the Kv1.1 PCR product revealed that this sequence was two bases shorter because of variations in the 3′ untranslated region.