Fig. 8.

Modulation by H₂O₂ of PMA-stimulated AP-1 (A) and NFκB (B) DNA binding. SH-SY5Y cells were incubated for 1 hr with 30–300 μm H₂O₂ followed by incubation for 2 hr with 1 μm PMA, and AP-1 and NFκB DNA binding were measured in nuclear extracts as described in Materials and Methods. Values are given as the percent of those obtained with PMA in the absence of H₂O₂ (as shown in Fig. 6). Mean ± SEM (n = 5). *p < 0.05 compared with cells treated with PMA alone (no H₂O₂) (ANOVA).