Table 1.

Effect of BSO on the reserpine-induced increase in glutathione content in PC12 cells

Treatment	Glutathione content (% respective control)
No drug	100  ± 3.4
Reserpine (50 nm)	222  ± 14.5*
BSO (100 μm)	100  ± 7.6
BSO (100 μm) + reserpine (50 nm)	276  ± 9.2*

After 2 d of culture, PC12 cells were rinsed free of medium and incubated for 3 hr at 37°C in a phosphate buffer (see Materials and Methods) in the absence of drugs or in the presence of reserpine alone, BSO alone, or the combination of BSO and reserpine, respectively. Data represent the mean ± SEM (n = 5–6) from two independent experiments and are expressed, in case of reserpine alone, as percentage of glutathione content after 3 hr in the absence of drugs and, in case of the combination of BSO and reserpine, as percentage of glutathione content after 3 hr in the presence of BSO alone. In the absence of drugs, the glutatione content after a 3 hr incubation amounted to 45.8 ± 3.2% from control at t = 0 hr, whereas in the presence of BSO glutathione content after a 3 hr incubation amounted to 26.7 ± 2.4% from control (mean ± SEM; p < 0.001 vs incubation in absence of drugs).

*p < 0.001 vs respective control.