Table 2.

Effect of treatment of PC12 cells for up to 3 d with reserpine on the GSH, DA, and DOPAC content

Treatment	Content (% respective control)
	GSH	DA	DOPAC
24 hr no drug	100  ± 12.3	100  ± 0.3	100  ± 0.8
24 hr reserpine (50 nm)	424  ± 2.82_a	1.3  ± 0.052_a	92.8  ± 1.12_b
48 hr no drug	100  ± 13.8	100  ± 1.5	100  ± 5.3
48 hr reserpine (50 nm)	296  ± 5.52_a	1.4  ± 0.052_a	85.4  ± 1.0
72 hr no drug	100  ± 8.3	100  ± 0.9	100  ± 1.5
72 hr reserpine (50 nm)	414  ± 1.72_a	1.4  ± 0.022_a	88.0  ± 1.22_c

After an initial 24 hr drug-free culture period, without a change of medium (see Materials and Methods), PC12 cells were kept in culture for an additional 24, 48, or 72 hr period in the absence (no drug) or presence of 50 nm reserpine, respectively. At the end of each incubation period, samples were prepared for determination of GSH, DA, and DOPAC content in parallel cultures, respectively, as detailed in Materials and Methods. Subsequent to preparation, all samples were stored at −20°C to ensure that determination of GSH, DA, and DOPAC content would take place on the same day. Data represent the mean ± SEM of a typical experiment performed in triplicate and are expressed as percentage of respective control (i.e., the value obtained for the respective incubation period in the absence of drug).

^F2_ap < 0.001 vs respective control.

^F2_bp < 0.01 vs respective control.

^F2_cp < 0.005 vs respective control.