Table 1.

Neuronal damage assessed by cell death, Ca²⁺overload, and mitochondrial depolarization

	20 min NMDA	50 min NMDA
Fraction of dead cells	0.39 ± 0.04 (23,601)	0.67 ± 0.06 (11,375)
Ca2+overload	0.13 ± 0.06 (107)	0.71 ± 0.06 (200)
Mitochondrial depolarization	0.37 ± 0.04 (74)	0.76 ± 0.02 (84)

The fraction of dead cells was calculated from counts of cultures stained with trypan blue 24 hr after treatments. Cell death in cultures treated with vehicle (0.07 ± 0.01; n = 18,846) was subtracted. Ca²⁺ overload was determined as in Figure1. Mitochondrial depolarization was derived as 1-Recovery Ratio (Fig.4). Values denote mean ± SEM; numbers in parentheses indicate the population of cells examined.