Fig. 4.

High-magnification photomicrographs of euploid (left) and Ts16 (right) cortices taken from the midpoint of the pallium, as shown in Figure 3A. At E13 (A), euploid and Ts16 cortices appeared similar in thickness of the VZ and PPL. At E16 (B), the size of the SP/CP, IZ, and pallium was smaller in Ts16 than in euploid brains. Moreover, the most dorsal cell-sparse region of the euploid IZ (immediately subjacent to the SP) was absent from the Ts16 cortex. By E18 (C), however, the thickness of all cortical layers appeared normal in Ts16 brains. PPL, Preplate;VZ/SVZ, ventricular zone/subventricular zone;ML, marginal layer; SP, subplate; CP, cortical plate; IZ, intermediate zone. Scale bar, 50 μm.