Fig. 5.

Effect of the inhibition of N-glycosylation, protein synthesis, and surface glycosyltransferases on the labeling of isolated dendrites with the tritiated sugar precursors mannose, galactose, and fucose. Neurites isolated as described in Materials and Methods were treated with tunicamycin (5 μg/ml), cycloheximide (50 μg/ml), or the impermeant sugar precursors UDP–galactose (5 mm) and pulse-labeled for 1 hr with tritiated sugars. Thebars represent the proportion of labeled and unlabeled MAP2-stained processes ± SEM after the different treatments. The numbers in parentheses indicate the number of dendrites evaluated in at least two different experiments. Controls were compared with the incorporation of [³H]leucine by isolated dendrites in similar conditions.