Fig. 3.
Subunit constituents of neuronal nicotinic receptors in the rat trigeminal ganglion. Immunoprecipitation of neuronal nicotinic receptors from rat trigeminal ganglion. Aliquots of [3H]-epibatidine-labeled, Triton X-100-solubilized rat trigeminal ganglion membranes equivalent to 30 mg of original tissue weight were incubated with rabbit antisera specific for each of the neuronal nicotinic receptor subunits indicated or normal rabbit serum (NRS) and precipitated with Pansorbin cells by centrifugation. Specific immunoprecipitation was calculated to be the difference between that obtained with each subunit-specific serum and that obtained with NRS. Data from three to seven experiments are expressed as the mean ± SEM in dpm/30 mg tissue.