Fig. 5.
Subunit composition of neuronal nicotinic receptor subtypes in the rat trigeminal ganglion. Double immunoprecipitation of neuronal nicotinic receptors from rat trigeminal ganglion. Aliquots of [3H]-epibatidine-labeled, Triton X-100-solubilized rat trigeminal ganglion membranes equivalent to 30 mg of original tissue weight were incubated with rabbit antisera specific for each of the neuronal nicotinic receptor subunits (indicated on theabscissa as 1st Antibody) or normal rabbit serum (NRS) and precipitated with Pansorbin cells by centrifugation. The resulting supernatant was reprecipitated in triplicate with a second antibody (indicated by theinset as 2nd Antibody). Data (mean ± SEM) are expressed as the percentage immunoprecipitated after the first antibody, which was calculated as the ratio of the amount of specific immunoprecipitation obtained by the second antibody after preclearing with the first antibody divided by the amount of specific immunoprecipitation obtained by the second antibody after preclearing with NRS (see text for rationale and interpretation). Data are representative of an experiment performed four times.