Fig. 4.

Ultrathin cryosections of type II neurons double-immunolabeled for ELH (small gold particles) and N-terminal peptide (large gold particles). Type I LDCV (arrowheads) were present in the Golgi (G) region (A, B), in clusters in the cell body (C), and in the axons (D). LEG were absent. Note that the intensity of ELH staining is highest in the axon (D). InD, a type II axon is closely opposed to a type I axon. A few type I LDCV are visible (arrows), which are larger and less intensely labeled for N-terminal peptide. N, Nucleus. Scale bars, 0.1 μm.