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. 1996 Nov 1;16(21):6742–6752. doi: 10.1523/JNEUROSCI.16-21-06742.1996

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Copyright © 1996 Society for Neuroscience

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Fig. 4. — Different isoforms of dynactin p150^Glued and the dynein IC74 and LICs are associated with SCb and the membranous organelles of anterograde fast component. A, The left pair of gel lanes (DYNACTIN) shows a comparison of the dynactin subunits associated with fast component (FC) and slow component b (SCb). The polypeptides are indicated to theright of the lanes: the p150^Glued and p135^Glued isoforms (p150/p135), p50 (p50), and Arp1 (p45). The right pair of gel lanes (DYNEIN) shows a comparison of the dynein subunits associated with fast component (FC) and slow component b (SCb). The dynein heavy chain (530), IC74 intermediate chain isoforms (IC74), and light intermediate chains (LIC) are indicated to the right of the lane. B, Isoforms of dynactin p150^Glued. C, Isoforms of dynein intermediate chains. D, Isoforms of dynein light intermediate chains. Lane 1, Analysis of the isoforms associated with the fast component (FC). Lanes 2, 3, Analysis of the isoforms associated with slow component b (SCb). Lane 2 was loaded with equal CPM of protein with respect to lane 1.Lane 3 shows the same sample as lane 2, except that the loading was increased to better visualize the more numerous isoforms associated with SCb.