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. 1996 Nov 1;16(21):6975–6986. doi: 10.1523/JNEUROSCI.16-21-06975.1996

Fig. 3.

Fig. 3.

Confocal photomicrographs showing localization of NK1-R (A–E) and NK3-R (F) in tissue sections and whole mounts. Tissues were incubated with NK1-R antiserum #94168 or NK3-R antiserum AP951.A, Section of antrum, showing localization of NK1-R in myenteric neurons. B, Section of jejunum, showing localization of NK1-R in myenteric neurons and interstitial cells of Cajal. C, Whole mount of the myenteric plexus of the ileum. D, Whole mount of interstitial cells of Cajal of the ileum. The arrows indicate prominent staining of the plasma membrane and endosomes. E, The sum of five optical sections of jejunum, collected at 0.36 μm intervals, showing localization of NK1-R in myenteric neurons and in interstitial cells of Cajal. F, An adjacent section to E (8 μm apart), the sum of five optical sections showing localization of NK3-R. The same neurons expressing the NK1-R express the NK3-R, whereas the interstitial cells are unstained.B–E show NK1-R-immunoreactive endosomes.mp, Myenteric plexus; cm, circular muscle. Scale bar (shown in F):A–D, 20 μm; E,F, 13 μm.