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. 1996 Nov 1;16(21):6713–6721. doi: 10.1523/JNEUROSCI.16-21-06713.1996

Fig. 2.

Fig. 2.

The retina contains a 35 kDa protein that binds to SNAP-25 but does not react with antibodies against syntaxin 1. Syntaxin 1-depleted (−) and nondepleted (+) retina and brain extracts were immunoprecipitated with a SNAP-25 antibody. The SNAP-25 immunoprecipitates are from: 1, whole retina extract to which no SNAP-25 antibody was added; 2, I.P. buffer alone; 3, nondepleted retina extract; 4, syntaxin 1-depleted retina extract; 5, nondepleted brain extract; and 6, syntaxin 1-depleted brain extract.A, The precipitated proteins were separated by SDS-PAGE, and then the gel was stained with Coomassie blue. The position of the 35 kDa bands is indicated by *, the 25 kDa bands by **, and the 18 kDa bands by ***. The position of size markers are indicated on theleft. B, Samples3–6 were run on a duplicate gel, electrophoretically transferred to nitrocellulose, and immunoblotted with antibodies against syntaxin 1, SNAP-25, and synaptobrevin.