Fig. 8.

Number of Cajal–Retzius cells present in the stratum lacunosum-moleculare/molecular layer (number of cells per 25,000 μm² sample) in single organotypic hippocampal cultures (H), double hippocampal cocultures (H-H), and entorhinohippocampal cocultures (E-H) after 5–7 DIV (black bars), 10–15 DIV (hatched bars), and 17–21 DIV (open bars) (mean ± SEM). Significant differences compared with short incubation times (5–7 DIV) are found in entorhinohippocampal cocultures (**p ≤ 0.01; ANOVA, Scheffe’s test).