Table 1.
Transgenic C. elegans strains and their properties
| Plasmid | Array | Host genotype | Motility | Sensitivity for cholinergic reagents | |
|---|---|---|---|---|---|
| Trichlorfon (mm) | Levamisole (mm) | ||||
| – | – | unc-18(+) | wild-type | 0.01 | >0.30 |
| – | – | unc-18(e81) | unc | 0.20 | 0.01 |
| pRF4 | kEx9 | unc-18(+) | wild-type | 0.01 | >0.30 |
| pRF4 | kEx1 | unc-18(e81) | unc | 0.20 | 0.01 |
| punc-18; pRF4 | kEx2 | unc-18(e81) | wild-type | 0.02 | >0.30 |
| punc-18; pRF4 | kEx3 | unc-18(e81) | wild-type | 0.02 | >0.30 |
| punc-18; pRF4 | kIn2-1 | unc-18(e81) | wild-type | 0.01 | >0.30 |
| punc-18; pRF4 | kIn2-2 | unc-18(e81) | wild-type | 0.01 | >0.30 |
| pMunc-18-1; pRF4 | kEx4 | unc-18(e81) | wild-type1_a | 0.01 | >0.30 |
| pMunc-18-1; pRF4 | kEx5 | unc-18(e81) | wild-type1_a | 0.01 | >0.30 |
| pMunc-18-1; pRF4 | kIn4-1 | unc-18(e81) | wild-type1_a | 0.01 | >0.30 |
| pMunc-18-1; pRF4 | kIn4-2 | unc-18(e81) | wild-type1_a | 0.01 | >0.30 |
| pMunc-18-3; pRF4 | kEx6 | unc-18(e81) | unc | 0.20 | 0.01 |
| pMunc-18-3; pRF4 | kEx7 | unc-18(e81) | unc | 0.20 | 0.01 |
punc-18, pMunc-18-1, and pMunc-18-3 plasmids were injected intounc-18(e81) with the plasmid pRF4, which confers a dominant Roller phenotype. Extrachromosomal arrays that behave as unstable free duplications are named kEx, and stable arrays integrated into a chromosome are named kIn. The motility and the sensitivity for cholinergic reagents of unc-18mutants are affected by these transgenes.
Wild-type means slightly slower motility than the motility of wild-type animals carrying rol-6. Three larvae were put onto NGM containing 10 different concentrations of reagents (0, 0.01, 0.02, 0.04, 0.06, 0.08, 0.1, 0.15, 0.2, and 0.3 mm). The highest concentrations are shown in which animals were able to produce F2 progeny within 10 d in triplicate experiments.