α6 and α3 heterodimerize with β1 in CG neurons. β1-Containing integrin heterodimers were immunoprecipitated from surface-biotinylated CG cells with anti-β1 (W1B10) mAb coupled to protein-A Sepharose. Immunoprecipitates were visualized directly by chemiluminescent detection of HRP-streptavidin (lane 1-sample buffer only; lane 2, antibody-coupled Sepharose), then probed with anti-α6Ex (lanes 3, 4). Similar immunoprecipitates were probed with anti-α3Ex2 (ip, lane 5). Triton X-100 extracts of chick breast fibroblasts (CEF, lane 6) and whole ciliary ganglia (CG, lane 7) were also blotted as positive controls.Arrows indicate bands corresponding to α6 or α3. Numbers indicate Mr in kilodaltons.
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