Fig. 2.

Effects of intracellular pressure (A), lanthanides (B), and salicylate (C, D) on the relationship between V_pkCm andCm_pk. Each point shows the relationship between V_pkCm andCm_pk. Increasing pressure, extracellularly applied lanthanides, or salicylate reducesCm_pk. A,Cm_pk and V_pkCmunder two pressure conditions, −0.41 ± 0.26 (mean ± SE) and 0.64 ± 0.23 kPa. Increasing pressure caused a positive shift in V_pkCm. B,Cm_pk and V_pkCmbefore and during application of lanthanides (3 or 10 mm GdCl₃, n = 7; 1 mm LuCl₃,n = 3; 1 mmCeCl₃, n = 1). Extracellularly applied lanthanides caused a negative shift inV_pkCm with decreasingCm_pk in all cells tested (n = 11). Reversibility was dependent on concentration. C,D, Cm_pk andV_pkCm before and during application of 10 mm salicylate. Extracellularly applied salicylate reduced Cm_pk, although the direction of the shift in V_pkCm was variable. The data are plotted in separate graphs for clarity. Of 21 cells tested, 13 showed a positive shift in V_pkCm (C), and 8 cells showed a negative shift (D). Each symbol indicates a different cell. All treatments were done afterV_pkCm stabilized.