Fig. 9.

Properties of the mossy fiber-evoked EPSC in homozygous mutant mouse granule cells. A, The mossy fiber-evoked composite EPSC under control conditions [−60 mV, 0 Mg²⁺, bicuculline (10 mm)]. The AMPA receptor- and NMDA receptor-mediated components of the composite EPSC can be resolved independently by the application of d-AP5 (50 μm) to illustrate the fast, AMPA receptor-mediated component (B), or the application of CNQX (10 μm) and glycine (10 μm) after recovery fromd-AP5 to illustrate the NMDA receptor-mediated component (C). Insets in B andC are faster time-base records with the control trace overlaid. All traces are from the same NR2C −/− mouse granule cell (P17). In D, the pharmacologically isolated NMDA receptor-mediated EPSC is displayed at a slightly higher gain and slower time base to display the biexponential decay of the EPSC; fit is overlaid as a solid line.