Fig. 2.

Kinetics and pharmacology of NMDA-triggered neurodegeneration and NO_x formation.A, Kinetics of NMDA-triggered neurodegeneration. Cultures were exposed to NMDA (100 μm, 5 min) and allowed to recover for various periods of time before culture viability (filled circles) and NO_xaccumulation (open circles) were measured.B, C, Pharmacology of NMDA-triggered neurodegeneration and NO_x accumulation. Cultures were exposed to NMDA (100 μm, 5 min) in the presence or absence of nitroarginine (NO₂Arg, 100 μm), and allowed to recover for 24 hr in control medium or medium containing AP5 (100 μm), dizocilpine (10 μm), or nitroarginine (100 μm) before culture viability (B) and NO_x accumulation (C) were measured.D, E, Delayed neuronal rescue. Cultures were exposed to NMDA (100 μm, 5 min) and allowed to recover for various periods of time before addition of either nitroarginine (100 μm, D) or dizocilpine (10 μm, E). Culture viability was assessed after 24 hr. All values are mean ± SEM of two to three separate experiments (with at least 3 culture wells per treatment per experiment). *p < 0.01, **p < 0.001 from control groups.